ABSTRACT
A method was developed for the determination of bifenthrin in tea by ultra performance convergence chromatography ( UPC2 ) . The samples were extracted with petroleum ether and cleaned up with Waters Sep-Pak:Carbon NH2 , and then detected by UPC2 . The mobile phase was the mixture of supercritical CO2 and acetonitrile at a flow rate of 1. 5 mL/min. The separation was conducted on a column of ACQUITY UPC2 TM BEH (100 mmí3. 0 mm,1. 7 μm). The UV detector was set at a wavelength of 220 nm. The detection limit was 20 μg/L. The linear range of bifenthrin was 0. 32-10. 30 mg/L. The recoveries ranged from 88. 7% to 98. 2%. The relative standard deviations (RSD) were from 1. 4% to 2. 8%. The result showed that the UPC2 was more efficient, rapid and low-cost than GC-MS. The method can meet the testing requirements of bifenthrin in tea. The efficacy of UPC2 on testing the concentration of bifenthrin in tea was compared with GC-MS based on National Proficiency Testing and the results were satisfactory.
ABSTRACT
OBJECTIVE To investigate the drug-resistance status of Klebsiella pneumoniae(KPN) in mountain hospitals and provide the reference for rational application of antibiotics.METHODS Referring to Rules of Operation in Clincal Laboratory of Nationwide,146 KPN isolates were cultured and identified and the drug-sensitivity test was performed in our hospital.RESULTS 100% of KPN isolates were sensitive to imipenem and meropenem.The resistant rates to amikacin,piperacillin/tazobactam,cefoperazone/sulbactam and cefoxitin were all below 22.0%.And that to the other 11 antibiotics were higher and all above 30%.The detection rate of the extended-spectrum-?-lactamases(ESBLs) producing KPN was 28.8%.CONCLUSIONS The drug-resistance of KPN in mountain hospitals is obviously lower than that in general hospitals of developed cities.We should strengthen reasonable management of antibiotics to prevent and control the occurrence and dissemination of drug resistant strains.
ABSTRACT
OBJECTIVE To investigate drug resistance of hospital acquired lower respiratory tract infection with Gram-negative bacilli in grass-roots hospitals and to provide the scientific reference for clinically reasonable use of antibiotics. METHODS Referring to Nationwide Rules of Operation in Clinical Laboratory,the strains were isolated and identified. The antibiotic susceptibility test was performed by K-B method. RESULTS Among 199 strains of Gram-negative bacilli,the top 4 of isolating rate were Pseudomonas aeruginosa (PAE),Klebsiella pneumoniae (KPN),Acinetobacter baumannii (ACB) and Escherichia coli (ECO). The drug-resistance supervision in vitro manifested ascending tendency of drug resistance in four kinds of important pathogenic bacteria. The susceptibility of Enterobacteriaceae to imipenem and meropenem was 100%,while the resistance rate of PAE and ACB arrived at 8.0-10.0%. The detection rate of extensive-spectrum beta-lactamases (ESBLs) producing ECO and KPN was 29.2% and 30.6%,respectively. CONCLUSIONS The drug resistance of lower respiratory tract infection with Gram-negative bacilli in grass-roots hospitals show ascending tendency. We should strengthen the management of hospital infection.